anti desmoglein 2 Search Results


human desmoglein-2 antibody  (Bio-Techne corporation)
90
Bio-Techne corporation human desmoglein-2 antibody
Human Desmoglein 2 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal anti hdsg2 antibody 6d8  (Bio-Rad)
92
Bio-Rad mouse monoclonal anti hdsg2 antibody 6d8
Mouse Monoclonal Anti Hdsg2 Antibody 6d8, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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anti desmoglein 2  (Boster Bio)
94
Boster Bio anti desmoglein 2
Anti Desmoglein 2, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
anti desmoglein 2 - by Bioz Stars, 2026-02
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rabbit anti-desmoglein2  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology rabbit anti-desmoglein2
Rabbit Anti Desmoglein2, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-desmoglein2 - by Bioz Stars, 2026-02
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polyclonal guinea pig anti-k8/18  (Progen Pharmaceuticals)
90
Progen Pharmaceuticals polyclonal guinea pig anti-k8/18
Albatross is a keratin-binding protein. (A) Immunoblotting with affinity-purified rabbit anti-Albatross antibodies. Specific recognition of a polypeptide with a relative molecular mass of ∼130 kD, the putative size of Albatross, in HeLa and Caco-2 epithelial cells (middle). Preincubation of the antibody with the antigen selectively inhibited the immunoreactivity (right). CBB, a loading control (left). (B) Lysates prepared from the indicated cells were subjected to immunoblot analysis for Albatross. (C) Anti-Albatross antibody immunoprecipitants were subsequently immunoblotted with the indicated antibodies. Left and right lanes show immunoprecipitates with control rabbit IgG and anti-Albatross antibodies, respectively. With the indicated epithelial cells, Albatross, keratin 8, and keratin 18 immunoprecipitated as a complex. (D) Double staining of keratin 18 and Albatross. Albatross is discontinuously associated with the <t>K8/18</t> filament network in the indicated epithelial cells. Mag., hyper-magnification of regions indicated by arrowheads. (E) Immunoblotting for keratin 8 and 18, and the indicated junctional and cell-polarity proteins in control and Albatross knockdown A549 (A1050 and A1160) cells. Albatross knockdown cells were produced successfully in which the expression level of keratin 8 and 18, the indicated AJC-specific transmembrane and undercoat proteins, lateral proteins, and apical proteins were not reduced. α-tubulin was included as a loading control. (F) Double staining for Albatross and keratin 18 in control and Albatross knockdown A549 (Albatross KD) cells. In control cells, Albatross is localized at the apical side of cell–cell border, and keratin filaments form apical bundles, whereas in Albatross KD cells, keratin filaments were apparently dissociated from cell–cell borders (arrowheads). Top and bottom columns illustrate projections of x-y planes and z sections, respectively. Bars, 10 μm.
Polyclonal Guinea Pig Anti K8/18, supplied by Progen Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal guinea pig anti-k8/18/product/Progen Pharmaceuticals
Average 90 stars, based on 1 article reviews
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desmoglein 2 (dsg2) mouse monoclonal antibody  (OriGene)
94
OriGene desmoglein 2 (dsg2) mouse monoclonal antibody
Albatross is a keratin-binding protein. (A) Immunoblotting with affinity-purified rabbit anti-Albatross antibodies. Specific recognition of a polypeptide with a relative molecular mass of ∼130 kD, the putative size of Albatross, in HeLa and Caco-2 epithelial cells (middle). Preincubation of the antibody with the antigen selectively inhibited the immunoreactivity (right). CBB, a loading control (left). (B) Lysates prepared from the indicated cells were subjected to immunoblot analysis for Albatross. (C) Anti-Albatross antibody immunoprecipitants were subsequently immunoblotted with the indicated antibodies. Left and right lanes show immunoprecipitates with control rabbit IgG and anti-Albatross antibodies, respectively. With the indicated epithelial cells, Albatross, keratin 8, and keratin 18 immunoprecipitated as a complex. (D) Double staining of keratin 18 and Albatross. Albatross is discontinuously associated with the <t>K8/18</t> filament network in the indicated epithelial cells. Mag., hyper-magnification of regions indicated by arrowheads. (E) Immunoblotting for keratin 8 and 18, and the indicated junctional and cell-polarity proteins in control and Albatross knockdown A549 (A1050 and A1160) cells. Albatross knockdown cells were produced successfully in which the expression level of keratin 8 and 18, the indicated AJC-specific transmembrane and undercoat proteins, lateral proteins, and apical proteins were not reduced. α-tubulin was included as a loading control. (F) Double staining for Albatross and keratin 18 in control and Albatross knockdown A549 (Albatross KD) cells. In control cells, Albatross is localized at the apical side of cell–cell border, and keratin filaments form apical bundles, whereas in Albatross KD cells, keratin filaments were apparently dissociated from cell–cell borders (arrowheads). Top and bottom columns illustrate projections of x-y planes and z sections, respectively. Bars, 10 μm.
Desmoglein 2 (Dsg2) Mouse Monoclonal Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
desmoglein 2 (dsg2) mouse monoclonal antibody - by Bioz Stars, 2026-02
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Image Search Results


Albatross is a keratin-binding protein. (A) Immunoblotting with affinity-purified rabbit anti-Albatross antibodies. Specific recognition of a polypeptide with a relative molecular mass of ∼130 kD, the putative size of Albatross, in HeLa and Caco-2 epithelial cells (middle). Preincubation of the antibody with the antigen selectively inhibited the immunoreactivity (right). CBB, a loading control (left). (B) Lysates prepared from the indicated cells were subjected to immunoblot analysis for Albatross. (C) Anti-Albatross antibody immunoprecipitants were subsequently immunoblotted with the indicated antibodies. Left and right lanes show immunoprecipitates with control rabbit IgG and anti-Albatross antibodies, respectively. With the indicated epithelial cells, Albatross, keratin 8, and keratin 18 immunoprecipitated as a complex. (D) Double staining of keratin 18 and Albatross. Albatross is discontinuously associated with the K8/18 filament network in the indicated epithelial cells. Mag., hyper-magnification of regions indicated by arrowheads. (E) Immunoblotting for keratin 8 and 18, and the indicated junctional and cell-polarity proteins in control and Albatross knockdown A549 (A1050 and A1160) cells. Albatross knockdown cells were produced successfully in which the expression level of keratin 8 and 18, the indicated AJC-specific transmembrane and undercoat proteins, lateral proteins, and apical proteins were not reduced. α-tubulin was included as a loading control. (F) Double staining for Albatross and keratin 18 in control and Albatross knockdown A549 (Albatross KD) cells. In control cells, Albatross is localized at the apical side of cell–cell border, and keratin filaments form apical bundles, whereas in Albatross KD cells, keratin filaments were apparently dissociated from cell–cell borders (arrowheads). Top and bottom columns illustrate projections of x-y planes and z sections, respectively. Bars, 10 μm.

Journal: The Journal of Cell Biology

Article Title: The keratin-binding protein Albatross regulates polarization of epithelial cells

doi: 10.1083/jcb.200803133

Figure Lengend Snippet: Albatross is a keratin-binding protein. (A) Immunoblotting with affinity-purified rabbit anti-Albatross antibodies. Specific recognition of a polypeptide with a relative molecular mass of ∼130 kD, the putative size of Albatross, in HeLa and Caco-2 epithelial cells (middle). Preincubation of the antibody with the antigen selectively inhibited the immunoreactivity (right). CBB, a loading control (left). (B) Lysates prepared from the indicated cells were subjected to immunoblot analysis for Albatross. (C) Anti-Albatross antibody immunoprecipitants were subsequently immunoblotted with the indicated antibodies. Left and right lanes show immunoprecipitates with control rabbit IgG and anti-Albatross antibodies, respectively. With the indicated epithelial cells, Albatross, keratin 8, and keratin 18 immunoprecipitated as a complex. (D) Double staining of keratin 18 and Albatross. Albatross is discontinuously associated with the K8/18 filament network in the indicated epithelial cells. Mag., hyper-magnification of regions indicated by arrowheads. (E) Immunoblotting for keratin 8 and 18, and the indicated junctional and cell-polarity proteins in control and Albatross knockdown A549 (A1050 and A1160) cells. Albatross knockdown cells were produced successfully in which the expression level of keratin 8 and 18, the indicated AJC-specific transmembrane and undercoat proteins, lateral proteins, and apical proteins were not reduced. α-tubulin was included as a loading control. (F) Double staining for Albatross and keratin 18 in control and Albatross knockdown A549 (Albatross KD) cells. In control cells, Albatross is localized at the apical side of cell–cell border, and keratin filaments form apical bundles, whereas in Albatross KD cells, keratin filaments were apparently dissociated from cell–cell borders (arrowheads). Top and bottom columns illustrate projections of x-y planes and z sections, respectively. Bars, 10 μm.

Article Snippet: The following primary antibodies were used: monoclonal mouse anti-keratin 8 (Ks 8.7; Progen Pharmaceuticals), monoclonal mouse anti-keratin 18 (CY-90; Sigma-Aldrich), polyclonal mouse anti-pan keratin (Sigma-Aldrich), polyclonal guinea pig anti-K8/18 (Progen Pharmaceuticals), polyclonal guinea pig anti–desmoplakin 1 (Progen Pharmaceuticals), monoclonal mouse anti–desmoplakin 1 and 2 (Progen Pharmaceuticals), monoclonal mouse anti–ZO-1 (1; BD Biosciences), monoclonal rat anti–ZO-1 (BM173; Acris Antibodies, GmbH), monoclonal rat anti–E-cadherin (ECCD-2; EMD), monoclonal mouse anti-neurofilaments, monoclonal rat anti–platelet/endothelial cell adhesion molecule (anti-PECAM; CD31; BD Biosciences), monoclonal mouse anti–α-tubulin (B-5-1-2; Sigma-Aldrich), monoclonal mouse anti–claudin-2 (12H12; Invitrogen), monoclonal mouse anti–desmocollin-2/3 (7G6; Invitrogen), monoclonal mouse anti–desmoglein 2 (10G11; Progen Pharmaceuticals), monoclonal mouse anti–nectin-1 (CK8; Invitrogen), monoclonal mouse anti–β-catenin (14; BD Biosciences), polyclonal rabbit anti-ezrin (Millipore), rabbit anti-Par3 polyclonal antibody (provided by S. Ohno, Yokohama City University, Yokohama, Kanagawa, Japan; Millipore), monoclonal mouse anti-occludin (OC-3F10; Invitrogen), monoclonal rat anti–nectin-2 (502–57; HyCult Biotechnology), polyclonal rabbit anti-GFP (Santa Cruz Biotechnology, Inc.), polyclonal rabbit anti-PKCζ (Santa Cruz Biotechnology, Inc.), and polyclonal rabbit anti–glyceraldehyde 3-phosphate dehydrogenase (anti-GAPDH) conjugated to HRP (Abcam).

Techniques: Binding Assay, Western Blot, Affinity Purification, Immunoprecipitation, Double Staining, Produced, Expressing

Functions of keratins and Albatross–Par3 complexes. (A–C) The amounts of Albatross protein and mRNA were analyzed in both keratin 8 and keratin 18 (K8/18)-introduced SW13 cells. As a control, an empty vector was transfected. As loading controls, α-tubulin and GAPDH were used. Two independent experiments were performed. (A) Immunoblotting. In transiently K8/18-introduced SW13 cells, the amount of Albatross protein is elevated, along with the amount of keratin 18. (B) With stable lines, the same results were obtained. (C) RT-PCR. In K8/18-introduced SW13 cells, the mRNA level of K18 is elevated, but not that of Albatross. β-actin is included as an internal control. (D) Double staining for K8/18 and the indicated proteins: Albatross, AJC components of ZO-1 and afadin, and Par3. (top) In control cells, K8/18 is absent and only limited amounts of Albatross are apparent at cell–cell junctions. In stably K8/18-introduced SW13 cells, Albatross is well localized in cell–cell junctions compared with control cells. (middle and bottom) ZO-1, afadin, and Par3 similarly accumulated at the cell–cell borders in stably K8/18-introduced SW13 cells. (E) Immunostaining of stably K8/18-introduced SW13 cells transfected with control or Albatross siRNA. Note that ZO-1, afadin, and Par3 are reduced at cell–cell borders with knockdown of Albatross. (F) A model for the regulation of AJC and lateral domains with the Albatross–Par3 complex and keratins. Albatross–Par3 complexes regulate the formation of AJC and maintain lateral membrane identity. However, Par3 without Albatross regulates apical structures. Keratins stabilize Albatross, promoting the formation of AJC. Knockdown effects are also indicated. Bars, 10 μm.

Journal: The Journal of Cell Biology

Article Title: The keratin-binding protein Albatross regulates polarization of epithelial cells

doi: 10.1083/jcb.200803133

Figure Lengend Snippet: Functions of keratins and Albatross–Par3 complexes. (A–C) The amounts of Albatross protein and mRNA were analyzed in both keratin 8 and keratin 18 (K8/18)-introduced SW13 cells. As a control, an empty vector was transfected. As loading controls, α-tubulin and GAPDH were used. Two independent experiments were performed. (A) Immunoblotting. In transiently K8/18-introduced SW13 cells, the amount of Albatross protein is elevated, along with the amount of keratin 18. (B) With stable lines, the same results were obtained. (C) RT-PCR. In K8/18-introduced SW13 cells, the mRNA level of K18 is elevated, but not that of Albatross. β-actin is included as an internal control. (D) Double staining for K8/18 and the indicated proteins: Albatross, AJC components of ZO-1 and afadin, and Par3. (top) In control cells, K8/18 is absent and only limited amounts of Albatross are apparent at cell–cell junctions. In stably K8/18-introduced SW13 cells, Albatross is well localized in cell–cell junctions compared with control cells. (middle and bottom) ZO-1, afadin, and Par3 similarly accumulated at the cell–cell borders in stably K8/18-introduced SW13 cells. (E) Immunostaining of stably K8/18-introduced SW13 cells transfected with control or Albatross siRNA. Note that ZO-1, afadin, and Par3 are reduced at cell–cell borders with knockdown of Albatross. (F) A model for the regulation of AJC and lateral domains with the Albatross–Par3 complex and keratins. Albatross–Par3 complexes regulate the formation of AJC and maintain lateral membrane identity. However, Par3 without Albatross regulates apical structures. Keratins stabilize Albatross, promoting the formation of AJC. Knockdown effects are also indicated. Bars, 10 μm.

Article Snippet: The following primary antibodies were used: monoclonal mouse anti-keratin 8 (Ks 8.7; Progen Pharmaceuticals), monoclonal mouse anti-keratin 18 (CY-90; Sigma-Aldrich), polyclonal mouse anti-pan keratin (Sigma-Aldrich), polyclonal guinea pig anti-K8/18 (Progen Pharmaceuticals), polyclonal guinea pig anti–desmoplakin 1 (Progen Pharmaceuticals), monoclonal mouse anti–desmoplakin 1 and 2 (Progen Pharmaceuticals), monoclonal mouse anti–ZO-1 (1; BD Biosciences), monoclonal rat anti–ZO-1 (BM173; Acris Antibodies, GmbH), monoclonal rat anti–E-cadherin (ECCD-2; EMD), monoclonal mouse anti-neurofilaments, monoclonal rat anti–platelet/endothelial cell adhesion molecule (anti-PECAM; CD31; BD Biosciences), monoclonal mouse anti–α-tubulin (B-5-1-2; Sigma-Aldrich), monoclonal mouse anti–claudin-2 (12H12; Invitrogen), monoclonal mouse anti–desmocollin-2/3 (7G6; Invitrogen), monoclonal mouse anti–desmoglein 2 (10G11; Progen Pharmaceuticals), monoclonal mouse anti–nectin-1 (CK8; Invitrogen), monoclonal mouse anti–β-catenin (14; BD Biosciences), polyclonal rabbit anti-ezrin (Millipore), rabbit anti-Par3 polyclonal antibody (provided by S. Ohno, Yokohama City University, Yokohama, Kanagawa, Japan; Millipore), monoclonal mouse anti-occludin (OC-3F10; Invitrogen), monoclonal rat anti–nectin-2 (502–57; HyCult Biotechnology), polyclonal rabbit anti-GFP (Santa Cruz Biotechnology, Inc.), polyclonal rabbit anti-PKCζ (Santa Cruz Biotechnology, Inc.), and polyclonal rabbit anti–glyceraldehyde 3-phosphate dehydrogenase (anti-GAPDH) conjugated to HRP (Abcam).

Techniques: Plasmid Preparation, Transfection, Western Blot, Reverse Transcription Polymerase Chain Reaction, Double Staining, Stable Transfection, Immunostaining